- BECKMAN COULTER AMPURE XP MANUAL
- BECKMAN COULTER AMPURE XP SERIES
All other trademarks are the property of their respective owners. in the United States and other countries. Not intended or validated for use in the diagnosis of disease or other conditions.īeckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc.
Knowledgeable support for reagents, automation and methods all from a single vendorĪMPure XP Part Numbers and Kit Sizes PART #. Quick transition with ready-to-implement methods. Below is estimated hands-on time and total time, in minutes, for AMPure XP. BECKMAN COULTER AMPURE XP MANUAL
Note the most common ratio used for NGS library cleanup is 1.8x.ĪMPure XP is a flexible reagent suitable for manual or automated workflows. Traces show relative differences in peak height and recovery efficiency. Cleanups were performed using 1.8x (red), 0.9x (blue), 0.7x (green), and 0.6x (aqua) reagent to sample ratios as shown. Only AMPure XP provides predictable performance while maintaining efficient recovery proven by the relative difference between the reference and 1.8x ratio in the traces below.Īgilent TapeStation traces of sheared gDNA purified using Ampure XP, Reagent “O”, and Reagent “K”.
Predictable and consistent size selection. 
Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants AMPure XP is a flexible reagent suitable for manual or automated workflows. High recovery of amplicons, greater than 100 bp. Maximizing recovery, consistency, and speed to facilitate the entire NGS workflow, AMPure XP is optimized to meet the stringent needs of today’s genomic applications and to minimize the risk of losing important genetic information. Nucleic acid purification and cleanup are mandatory for genomic applications including sequencing, qPCR/ddPCR/PCR, microarrays and other enzymatic reactions. Plate Loader Options for the CytoFLEX PlatformĪMPure XP Manual or Automated Purification & Cleanup. BECKMAN COULTER AMPURE XP SERIES
CytoFLEX Violet-Blue-Red Series Upgrades. Labware for Liquid Handling Instruments. Lysing, Fixative, and Permeabilizating Reagents. RESOURCE Contract Manufacturing Services. Search Conjugated Antibodies, Antibody Cocktails. ANATEL A1000 TOC Analyzer for Ultrapure Water. HIAC 9703+ Pharmaceutical Particle Counter. HIAC 8011+ Industrial Laboratory System. Echo 650 Series Acoustic Liquid Handlers. Biomek NGeniuS Next Generation Library Prep System. Navios EX – Powerful, Dependable Flow Cytometer. Cell Counters, Sizers and Media Analyzers. Air Particle Counters for Cleanroom and Environmental Monitoring. This protocol is for demonstration only, and is not validated by Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. High-speed atomic force microscopy revealing contamination in DNA purification systems. “Our findings suggest AMPure XP would be the best choice for analyses requiring very high analytical stringency.” Mikheikin, A., Olsen, A., Picco, L. Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants. High recovery of amplicons greater than 100bp. Maximizing recovery, consistency, and speed, AMPure XP meets the stringent needs of today’s genomic applications and minimizes the risk of losing important genetic information. Nucleic acid purification and cleanup are mandatory for genomic applications, such as sequencing, qPCR/ddPCR/PCR, and microarrays. Over 15,000 publications used AMPure XP, referenced in articles in Science, Nature, and PNAS. 
Over 200 library prep kits suggest using AMPure XP, including kits from trusted sequencing companies:
Plate Loader Options for the CytoFLEX PlatformĪMPure XP is the Gold Standard for Bead Based, Next-Generation Sequencing (or NGS) Cleanup.
0 kommentar(er)
